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KMID : 0380020090240020189
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Korean Journal of Biotechnology and Bioengineering
2009 Volume.24 No. 2 p.189 ~ p.194
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Enhanced effect extraction of Antioxidant substance Homoorientin from Pseudosasa japonica and Phyllostachys bambusoides leaves using Ultrasonic wave system
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Lee Kwang-Jin
Shin Yong-Kook
Kim Young-Sik
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Abstract
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In this work, antioxidant substance homoorientin from Pseudosasa japonica and Phyllostachys bambusoides leaves wereextracted using ultrasonic system, and analyzed by reversed-phase high performance liquid chromatography (RP-HPLC) on-line ABTS (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) antioxidant screening method. Also, the various experimental variables were the frequency and time of ultrasonic system. From the results, homoorientin was high extracted at the experimental condition of low frequency 35 kHz and time 60 min. And the content of homoorientin in Phyllostachys bambusoides was remarkably higher than that in the Pseudosasa japonica extract.
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KEYWORD
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Bamboo, Flavone C-glycosides, Homoorientin, Ultrasonic wave, HPLC
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